The isolation of platelets from platelet-rich plasma (PRP) is a critical step in various medical applications, including tissue engineering, wound healing, and regenerative medicine. The use of acoustic forces to separate platelets from PRP offers a non-invasive and efficient approach to achieving this goal. Acoustic radiation forces mobilize cells in flow according to their volume and density without contact and pressure.
Gene editing using lentiviral vectors has become a compelling and safe method in cellular therapy specifically for T-cells through the expression of a chimeric antigen receptor. However, the efficiency of the gene transfer to the T-cells has remained a burden. Another limitation of this very approach is that these viral vectors are hard to provide, thus expensive and conventional transduction methods exhibit a high multiplicity of infection, i.e. the viral vectors are used in large excesses compared to cells. Therefore, we developed an acoustophoresis system to enhance the transduction efficiency of T-lymphocytes using minimum viral particles to
cells with acoustophoresis Acoustophoresis is an ultrasound based technique to replace most cell sorting and washing devices such as centrifuges. It uses low acoustic radiation forces to make cells migrate to a pressure node. Unlike a centrifuge, Aenitis’ technology works completely in flow and can be part of a more complex fluidic system. It can be used to sort, wash and concentrate all kinds of cells and tissues, with no change in cell viability. Flow rates can range from 0.5 ml/min to 40 ml/min. Previous results have shown that acoustophoresis can be used safely on
Acoustophoresis is an emerging technology allowing cell separation based on their physical properties via ultrasound. The compressibility and density of the cell and its surrounding mediumdefine an acoustic contrast factor (ACF), which governs the migration direction of the cell. Different cell types with distinct ACFs would be separable by acoustophoresis. This innovative methodhas the advantages to be non-contact, label-free, and working in a controlled flow. Those features make it a promising solution for any bioproduction process requiring isolation and processingof cells without the disadvantages of centrifugation or immunomagnetic separation. It has already been successfully used
These last decades have seen the emergence and development of cell-based therapies, notably those based on mesenchymal stromal cells (MSCs). The advancement of these promising treatments requires increasing the throughput of processed cell for industrialization in order to reduce production costs. Among the various bioproduction challenges, downstream processing, including medium exchange, cell washing, cell harvesting and volume reduction, remains a critical step for which improvements are needed. Typically, these processes are performed by centrifugation. However, this approach limits the automation, especially in small batch productions where it is performed manually in open system. https://doi.org/10.1016/j.jcyt.2023.05.003
Les forces de radiations acoustiques mobilisent les cellules en suspension avec une intensité sélective selon leur volume et leur densité en l’absence de contact et de pression. L’acoustophorèse utilise ce principe pour la préparation de concentrés plaquettaires (CP). https://doi.org/10.1016/j.tracli.2021.08.036
Purity, limited platelet activation, and preservation of platelet function are important stakes of preparation of platelet concentrates (PC) for clinical use. In fact, contaminating red blood cells and leukocytes, as well as activated and/or poorly functional platelets in PC, represents a risk of poor efficiency and adverse side effects during platelet transfusion. Therefore, optimization of preparation and storage of PC is still an active field of research. Shear-induced platelet activation is an unwanted side effect of the hard-spin (up to 5000g) step of centrifugation-based methods currently used in blood banks to prepare PC from whole