Novel method to enhance T-lymphocyte viral transduction by acoustophoresis

Gene editing using lentiviral vectors has become a compelling and safe method in cellular therapy specifically for T-cells through the expression of a chimeric antigen receptor. However, the efficiency of the gene transfer to the T-cells has remained a burden. Another limitation of this very approach is that these viral vectors are hard to provide, thus expensive and conventional transduction methods exhibit a high multiplicity of infection, i.e. the viral vectors are used in large excesses compared to cells.  Therefore, we developed an acoustophoresis system to enhance the transduction efficiency of T-lymphocytes using minimum viral particles to address both concerns of transduction quality and quantity of viral vectors. Acoustophoresis is a contactless and soft method to process the cells while controlling the flow precisely.