Acoustic Transduction & Transfection Boost System (A2TB-S)
CAR-T cell therapy has shown remarkable success in the treatment of various cancers. However,
producing these cells for clinical use is still challenging. One of the significant challenges in CAR-T
cell production is transducing the cells with lentivirus vectors efficiently. Low transduction efficiency
results in lower CAR expression and reduced cytotoxicity, ultimately affecting the efficacy of the
therapy. This poster presentation discusses the use of acoustic technology to enhance the
transduction efficiency of CAR-T cells. Benefits of using acoustic to enhance transduction: Acoustic
technology has recently emerged as a promising approach for enhancing the transduction efficiency
of CAR-T cells. Acoustic technology uses sound waves to create a membrane rearrangement and
permeability in the target cells. Cell membrane transient alteration allowing efficient vector delivery
without causing cell damage or toxicity. Furthermore, the acoustic method does not require any
additional reagents or steps, making it a cost-effective and time-efficient approach.
The objective of the Acoustic Transfection & Transduction Boost System (ATTB-S) is to demonstrate the boosting effect of acoustic stimulation on the transduction of T lymphocytes by retro/lentivirus.