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The isolation of platelets from platelet-rich plasma (PRP) is a critical step in various medical applications, including tissue engineering, wound healing, and regenerative medicine. The use of acoustic forces to separate platelets from PRP offers a non-invasive and efficient approach to achieving this goal. Acoustic radiation forces mobilize cells in flow according to their volume and density without contact and pressure.

Gene editing using lentiviral vectors has become a compelling and safe method in cellular therapy specifically for T-cells through the expression of a chimeric antigen receptor. However, the efficiency of the gene transfer to the T-cells has remained a burden. Another limitation of this very approach is that these viral vectors are hard to provide, thus expensive and conventional transduction methods exhibit a high multiplicity of infection, i.e. the viral vectors are used in large excesses compared to cells.  Therefore, we developed an acoustophoresis system to enhance the transduction efficiency of T-lymphocytes using minimum viral particles to

These last decades have seen the emergence and development of cell-based therapies, notably those based on mesenchymal stromal cells (MSCs). The advancement of these promising treatments requires increasing the throughput of processed cell for industrialization in order to reduce production costs. Among the various bioproduction challenges, downstream processing, including medium exchange, cell washing, cell harvesting and volume reduction, remains a critical step for which improvements are needed. Typically, these processes are performed by centrifugation. However, this approach limits the automation, especially in small batch productions where it is performed manually in open system. https://doi.org/10.1016/j.jcyt.2023.05.003

Acoustophoresis is an emerging technology allowing cell separation based on their physical properties via ultrasound. The compressibility and density of the cell and its surrounding mediumdefine an acoustic contrast factor (ACF), which governs the migration direction of the cell. Different cell types with distinct ACFs would be separable by acoustophoresis. This innovative methodhas the advantages to be non-contact, label-free, and working in a controlled flow. Those features make it a promising solution for any bioproduction process requiring isolation and processingof cells without the disadvantages of centrifugation or immunomagnetic separation. It has already been successfully used