Aenitis develops a technology that uses the acoustic strength (ultrasonic waves) generated between the walls of a microchannel in order to separate and manipulate without contact blood elements such as particles or cells.This video shows on a large scale the effects of concentration by acoustic waves on diluted red blood cells (without flow, in real time).
How does it work?
Ultrasonic waves generate acoustic radiation forces (ARF) between the walls of a microchannel. Indeed, provided to comply with a resonance condition on the acoustic wavelength, this technology enables the creation of an acoustic pressure node right in the center of the channel. The ARF then push the particle towards the pressure node with a force of up to a hundred times gravity equivalent. The objects suspended in the fluid between the two walls will then migrate to the sound pressure node and can then remain trapped in this position. With this approach, it is possible to levitate objects of sizes ranging from 1 μm to several tens of microns in micro-channels of hundreds of μm thick. The ARF acting on the suspended objects is highly dependent on physical properties (such as density) and geometric. Thus, depending on the amplitude of the sound wave, we can force some species to cluster at the center channel while others cannot be displaced because of too low ARF.
Illustration of the acoustic focalisation principle of suspended particles towards the center of the channel
Benefits of Aenitis' technology:
- No mechanical contact between cells
- Very little shearing and pressure strengths
- Continuous flow treatment (microchannel drain)
This approach represents a breakthrough innovation compared to the “traditional” techniques (such as centrifugation).
Depending on the chosen designs and parameters, Aenitis' technology enables to separate cells from platelets, thus producing high quality platelet concentrate.
The current results of the study are very promising (effective separation between cells and platelets) and are being optimized (improvement of the separation and the flow rates) in order to meet blood banks’ production needs.
The ability to test Aenitis prototypes in real conditions, and to take into account blood banks’ functional and operational constraints at a very early stage, is essential. Machines answer to the very strict standards while upgrading the production quality and simplifying its process.